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Cryo-EM sample preparation and data collection Sucrose gradient purified fractions of the PV3-SC8 or wt PV2 VLPs expressed in yeast and mammalian cells, respectively, were pooled, buffer exchanged into DPBS-EDTA using Zeba Spin Desalting Columns (Thermo Fisher Scientific) with a 7 K molecular weight cut-off (MWCO) and concentrated using Amicon Ultra centrifugal filter devices (100 kDa MWCO, Merck Millipore) to a concentration of 0.91.5 mg/ml

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The anti-oxidant effect of -OHB could be also related to the ability of ketone bodies to oxidize co-enzyme Q (Sato et al., 1995

10.3390/pharmaceutics14030576 [DOI] [PMC free article] [PubMed] [Google Scholar] 100.Balestrino R, Schapira AHV

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