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If at any time the patient develops any severe symptoms (Table 1) after the first dose, administer two additional DuoDote injections i.m

The samples were transferred quantitatively onto spin columns (BioEcho) and washed as follows: 0.4% SDS in PBS (3 700 l), 6 M urea in ddH 2 O (2 700 l) and PBS (3 700 l) and transferred into new 1.5-ml LoBind tubes (Eppendorf) with 2 100 l X buffer (7 M urea, 2 M thiourea in 20 mM HEPES, pH of 7.5)

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[DOI] [PubMed] [Google Scholar] 49.Hgberg L., Webb C., Flth-Magnusson K., Forslund T., Magnusson K.E., Danielsson L., Ivarsson A., Sandstrm O., Sundqvist T