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MICU1 imparts the mitochondrial uniporter with the ability to discriminate between Ca 2+ and Mn 2+

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For GFP labeling and the generation of translational fusion constructs, the mrp-1::gfp cassette was expressed using 3 kb of the promoter region of mrp-1 or 3.3 kb of the promoter region of the intestine-specific gene ges-1 to generate the pJL2 (pPD95.75_P mrp-1::mrp-1::gfp ) and pJL3 (pPD95.75_P ges-1::mrp-1::gfp ) plasmids, respectively