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Relevance of molecular docking studies in drug designing

Plasmids Codon optimized cDNA encoding EBV gH (GenBank AFY97969.1) and gL (GenBank: AFY97944.1) separated by a furin cleavage site and a porcine teschovirus-1 (P2A) ribosomal skipping peptide in both orientations (gH-furin-P2A-gL or gL-furin-P2A-gH) with a 5 Kozak consensus sequence were synthesized by Twist Biosciences and cloned into pVEE-rep encoding the 5 and 3 untranslated regions and the nonstructural open reading frame of Venezuelan equine encephalitis virus, strain TC-83, between PflFI and Sac II sites 42,73 , creating pVEE-gH-gL and pVEE-gL-gH

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However, the relationship between the presence of these typical markers of apoptosis in spermatozoa and the degree of DNA fragmentation is not straightforward

Lane, T