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211 The NLRP3 protein acts as a sensor for the mature inflammasome and is responsible for assembling the classical inflammasome during inflammatory stimulation

The flow-through fraction containing the Fab fragments was collected, while the Fc fragments were eluted using a high-salt buffer (2.2 mM PBS supplemented with 500 mM NaCl)

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Kobayashi, Y., Tokuda, K., Yamashiro, C., Higashijima, F., Yoshimoto, T., Ota, M., Ogata, T., Ashimori, A., Hatano, M., Kobayashi, M., Uchi, S