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Third generation sequencing is performed through single-molecule real-time (SMRT) sequencing provided by PacBio, which is able to analyze the kinetics of polymerase [49], fulfilling an impactful role in genetic disorder diagnosis, especially for repetitive sequences-associated diseases, where short-read sequencing is not able to provide correct diagnoses through technical limitations (the length of the reads could not be sufficient for mapping repetitive regions longer than the respective read) [52]

10.1016/0006-291X(89)91060-7 63 GeierA.KimS

10.1016/S0021-9673(02)01971-4 109 LarreyD

All animal experiments were approved by and conducted in compliance with Institutional Animal Care and Use Committee (IACUC) of the University of Colorado Anschutz Medical Campus

(Siegel et al., 2020)