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In our study, we observed that BDG increased glutathione levels in monocytes and that intracellular GSH was able to reduce ROS production post-translationally

Another study further tests the correlation between GSH depletion and patients with MCI and results in a similar conclusion (Lin and Lane, 2021)

Western Blot After the drug treatment, the proteins from the cultured SGNs were extracted with radioimmunoprecipitation assay buffer (RIPA) buffer (Protein Biotechnology, China)

van Bergen JM, Li X, Hua J, Schreiner SJ, Steininger SC, Quevenco FC, et al

FIGURE 5 To investigate the fusion of mitophagosomes with lysosomes, which is the final step of mitophagy, MitoTracker and LysoTracker probes were used to label mitochondria and lysosomes, respectively