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Synergy was defined as a 2 log10 CFU/mL reduction at 24 h in a combination when compared to the CFU/mL of the most active individual drug ( 2.5 Total ROS assay 2,7-dichlorofluorescein diacetate (DCFH-DA) dye was used to measure reactive oxygen species (ROS) levels in CRKP as previously described ( 2.6 Bacterial metabolite extraction Metabolite extraction was performed as described previously with a few modifications ( 2.7 LCMS/MS analysis and data preprocessing A UHPLC system (Vanquish, Thermo Fisher Scientific) with a UPLC HSS T3 column coupled to an Orbitrap Exploris 120 mass spectrometer (Orbitrap MS, Thermo) was employed to perform LCMS/MS analysis

This higher level formula is especially indicated for animals off feed due to shipping, illness, or other stress factors

10.3389/fcell.2019.00262 25 NavarreteF

doi: 10.1080/14728214.2017.1269744

limited data exists beyond 12 weeks