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We found that the full-length human GCLC transcript was cleaved by recombinant human IRE1 in vitro, which can be inhibited by 48c, a potent IRE1 RNase inhibitor 19 (Fig

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1991;54:909) 82 3 The Micronutrients References mental retardation in the world.2 Despite widespread efforts to iodize salt, IDD con- 1

The binding reactions were incubated and resolved by native polyacrylamide gel electrophoresis as described for 6-FAM-ARE 19 , except the binding reactions were resolved on a 6% (w/v) polyacrylamide/1X TB gel, and the gel was exposed to storage phosphor screen which was then scanned using an Amersham Typhoon Biomolecular Imager (Cytiva) on the phosphorimager setting