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Second, we did not measure sputum consistency or volume, which are essential factors for validating the effect of sputum expectoration

The membrane was washed three times in Tris-buffered saline with Tween 20 (TBS-T) for 10 min, and treated with HRP-conjugated anti-rabbit or anti-mouse IgG (GE Healthcare) for 1 h

On the other hand, it efficiently delivers p65 siRNA to target and silence NF-B, thus reducing hepatocyte injury markers (ALT/AST), suppressing the release of inflammatory factors, and significantly ameliorating acute liver injury by targeting this central inflammatory pathway (Yin et al., 2023)

How I match the protocol to the procedure The protocol is not a single template

Despite the lack of difference in immune response during the acute phase of infection in this study, increased dietary arginine did significantly improve growth efficiency in the challenged birds