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All samples were desalted and concentrated using Oasis HLB 3cc (60 mg) reversed-phase cartridges (Waters, Milford, MA, USA) (elution with 1.5 mL of methanol containing 0.5% FA)

Hubbard BP, Gomes AP, Dai H, Li J, Case AW, Considine T et al

As demonstrated in Figure 2, this novel senolytic did remove cells from the PDL9 chondrocytes, which was accompanied by significantly reduced levels of SA--gal staining

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