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Supplementary Tables (download XLSX Supplementary Table 2

International Journal of Molecular Sciences, 25(3), 1459 .

IGF-1 then helps regulate GH release

Quick Notes Some peptides are harder to dissolve this is normal, just gently swirl and let it sit for 510 minutes before use

Co-immunoprecipitation (Co-IP) assay Cells after indicated treatments were lysed in IP buffer (50 mM Tris-HCl, pH 7.5, 0.5% NP-40 (MF430, Mei5bio), 150 mM NaCl, 5 mM EDTA, 10% Glycerin) with protease inhibitor cocktail (B14001, Bimake) for 30 min at 4 C, followed by the enrichment of target proteins using Flag-M2 beads (B23102, Bimake) or specific antibodies overnight at 4 C