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Cross-regulation of signal pathways: Study the interaction between AOD 9604 and other signal pathways (such as AMPK, mTOR)

The BL21 transformants were plated on LB agar plate with kanamycin (KAN) (30 g/mL) and incubated overnight at 37 C

Statements Data availability statement The original contributions presented in the study are included in the article/ Supplementary Material

5-Amino-1MQ (5-amino-1-methylquinolinium) is the most studied inhibitor of nicotinamide N-methyltransferase (NNMT) an enzyme that acts as a metabolic brake in fat cells by depleting both SAM (S-adenosylmethionine) and NAD+ precursors

The naturally occurring phytocannabinoids can be extracted from the plant and isolated, or they could be synthesized in a lab