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*Correspondence: Luclia Domingues, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal e-mail: [email protected] This article was submitted to Microbiotechnology, Ecotoxicology and Bioremediation, a section of the journal Frontiers in Microbiology

The slides were rinsed once with phosphate buffer saline (PBS) and ultra V protein block was applied and incubated for 10 min

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Most online discussions mention different approaches, but there is no universal standard