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Electroporation was carried out using a MicroPulser Electroporator (BIO-RAD) at 1.8 kV

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Owing to their excellent specificity, low-toxicity, rich chemical diversity and availability from natural sources, FDA has successfully approved a number of peptide-based drugs and several are in various stages of drug development

Next, 160 L of the extract (acetonitrile-water volume ratio: 1:1) was added before samples were vortexed for 30 s, sonicated for 10 min in an ice-water bath, and centrifuged at 4C for 15 min at 12,000 rpm

doi: 10.1007/978-3-642-00390-5_5 14 BegcyK.MarianoE