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For glucose starvation, media with normal glucose level were removed, cells washed twice with 1X PBS, and glucose-deprived media (final glucose level at 0.5 mM) with or without lactic acid added for 12 h

These are unstable molecules produced by UV radiation, air pollution, and even intense physical exercise

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For treatment, they offer drug targets, and understanding them aids in optimizing existing drugs and personalized therapy

As in the case of many chronic degenerative diseases, increased production of reactive oxygen species has been considered to play an important role, even in the pathogenesis of chronic fluoride toxicity