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24-26 Isolation, Screening and extraction of bioactive metabolites After the serial dilution the sample were plated on the plate, among the different isolates obtained through agar overlay assay which indicate the antibacterial property of the isolate (Figure 1)

The BIOM file output from the DADA2 pipeline and the DECIPHER-aligned FastTree phylogenetic tree were input into QIIME (v1.9.1) and the scripts in the Supplementary Materials (QIIME Scripts compilation) were used to establish the alpha diversity metrics of Chao1 richness estimate, Simpsons evenness measure E, Shannon diversity index H, Simpsons diversity index, and Faiths phylogenetic diversity metric (whole tree) as well as the beta diversity measures of unweighted and weighted UniFrac analyses (the latter of which factors in ASV abundance 37 ) and BrayCurtis dissimilarity analysis

[DOI] [PMC free article] [PubMed] [Google Scholar] 54.Bromelain a potential bioactive compound: a comprehensive overview from a pharmacological perspective

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