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To isolate the barcode and IS, we allowed 3 mismatches in the 23 bp invariant sequence upstream and up to 6 mismatches in the 50 bp downstream of the barcode using a Hamming distance function to compare the expected and actual sequences

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Yes, to some extent this is similar to how a specific J1939 parameter group (PG) will contain multiple SPNs/signals (aka data parameters) in the 8 data bytes