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A comprehensive pre-treatment panel typically includes a Complete Metabolic Panel (CMP) to assess liver and kidney function, a Complete Blood Count (CBC), thyroid function tests (TSH, Free T3, Free T4), fasting glucose and insulin levels, HbA1c, and a lipid panel

All these amino acids are necessary for protein synthesis and collagen formation

The transcript levels of these reference genes did not differ between the experimental groups (Supplementary Figure 1)

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To clarify the specific mechanism by which DON induces oxidative damage to granulosa cells, this study used proteomics to detect the protein expression levels before and after DON treatment