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Culture density was monitored using a Genesys 150 UV-Vis spectrophotometer (Thermo Fisher Scientific, Waltham, MA) at a wavelength of 600 nm

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Accordingly, it is possible that mitochondrial complex IV is more vulnerable to moderate HIF1 accumulation, as in aged adipocytes, and more robust HIF1 accumulation (as in Vhl -deficient cells) is necessary to simultaneously suppress complex I, II and IV by the mechanisms described above