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As an outlook, the simultaneous inhibition of essential oxidative stress response enzymes by the 5-nitroimidazole ether analogues causes a perfect storm of damage and attenuated rescue (Fig

3.4.3 Incentive mechanisms for the adaptation of FBS alternatives The reduction and replacement of FBS use with alternatives are becoming feasible as hPL and other serum-free substitutes have demonstrated performance comparable to that of FBS, supporting MSCs proliferation without compromising phenotypic stability, and enhancing monoclonal antibodies production in hybridoma cell lines (Even et al., 2006

It is difficult at this stage to establish a causal sequence of events that underlie these observations

Others, such as corynoxine, target upstream phosphatases such as protein phosphatase 2A to dually inhibit AKT signaling (98), whereas Gboxin and its analog Y9 trigger lysosomal dysfunction and autophagic impairment (99)

In addition, we have performed proof-of-principle experiments that such experimental models will prove useful in the discovery and validation of molecularly targeted therapies