estimation of tissue reduced glutathione Rapid Quantification Oxidized and Forms of Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro Determination of Reduced and Total

& Woodgett, J

2.8 Immunohistochemical analysis Paraffin sections from different groups of liver tissues from rats were stained by immunohistochemistry (IHC) according to the manufacturing protocol using Anti-caspase 3 antibody (ab4051), at dilution 1:1,000, Anti-Bax antibody (ab32503), at dilution 1:250 and Anti-Bcl-2 antibody (ab182858), at dilution 1:500, (abcam, Cambridge, United Kingdom)

3 Con A-induced hepatitis model is a well-established experimental murine model mimicking human T cell-mediated liver disease

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Consonantly, when we compared the Uvaol + GBS group with the GBS group, from the 20 changed Raman bands, uvaol prevented the alterations from 11 bands (725, 729, 915, 1,004, 1,028, 1,041, 1,091, 1,096, 1,098, 1,662, and 1,667 cm 1 ), with a synergic effect of only one band (788 cm 1 )