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BPC-157 is believed to interact with the nitric oxide (NO) system, angiogenic growth factors, and cellular signaling pathways like FAK-paxillin and VEGF, which are involved in cell migration, cytoskeletal reorganization, and vascular modulation

Because the assembly and amplification reactions are in vitro enzymatic reactions, they can be carried out sequentially in microplates and without cells, making them amenable to high-throughput screening applications

The IC 50 values of the synthesized compounds deviated significantly from that belonging to the reference compounds, with the 5-SH analogues 32 and 34 as the most active