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Each reaction contained 1X of reaction buffer, 1X of stabilizer solution, 0.2 mM dNTPs each, 1 M of each primer (with FAM and HEGL), 30 L of the ssDNA pool recovered from the selection, and 1.25 U NeoProof DNA Polymerase in a total volume of 50 L

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p38 MAPK signaling pathway was not involved in the regulation of mt UPR activation in simulated microgravity treated nematodes Our previous study has raised an intestinal cascade of NSY-1-SEK-1-PMK-1-ATF-7/SKN-1 required for the control of simulated microgravity toxicity in nematodes 24

Boumezbeur F, Mason GF, de Graaf RA, Behar KL, Cline GW, Shulman GI, Rothman DL, Petersen KF (2010) Altered brain mitochondrial metabolism in healthy aging as assessed by in vivo magnetic resonance spectroscopy