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10.18433/j3nc79 122 PivinaL.SemenovaY.DoaM

Chronic beryllium disease, which presents with granulomatous inflammation resembling sarcoidosis, arises from an immune hypersensitivity reaction to beryllium [61]

What diluent should be used

Foerster EG, Mukherjee T, Cabral-Fernandes L, Rocha JDB, Girardin SE, Philpott DJ

After sequential washes with low salt buffer (20 mM, Tris pH 8.0, 2 mM EDTA, 150 mM NaCl, 1% Triton, 0.1% SDS), high salt buffer (20 mM Tris pH 8.0, 2 mM EDTA, 500 mM NaCl, 1% Triton, 0.1% SDS), LiCl buffer (10 mM Tris pH 8.1, 0.25 M LiCl, 1 mM EDTA, 1% NP40, 1% IGEPAL) and TE buffer, the DNA-bound immunocomplexes were eluted and the DNA were extracted by PCR clean up kit