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In eukaryotes, initiation complex formation is similar, with the following differences: The initiator tRNA is a different specialized tRNA carrying methionine, called Met-tRNAi Instead of binding to the mRNA at the Shine-Dalgarno sequence, the eukaryotic initiation complex recognizes the 5 cap of the eukaryotic mRNA, then tracks along the mRNA in the 5 to 3 direction until the AUG start codon is recognized

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