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Splenocytes from challenged mice were stimulated only with PMA and Ionomycin for 4 h in the presence of Golgi Plug and Stop Golgi

196 Although the mutation frequency of E3 ubiquitin ligase is lower than that of other WNT inhibitors, mutations of RNF43 (which mediates ubiquitin degradation of Frizzled) are still detected in the adenoma tissues of patients with GC, 218 early-onset metastatic CRC, 219 and Lynch syndrome