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Upon confluence of the seeded HCE-2 cells in the culture flask, the cells were trypsinized with Trypsin-EDTA, then trypsinization was stopped with a complete growth medium and cells were harvested, counted and seeded into the six prepared wells

In addition, ExerVs injected during disuse accelerated the ability for Type I oxidative fibers, which are most susceptible to atrophy 21 , to rapidly recover size during the reloading period

Roma-Rodrigues C, Mendes R, Baptista PV, Fernandes AR (2019) Targeting tumor microenvironment for cancer therapy

KPV is a tri-peptide composed of three amino acids lysine, proline, and valine derived from alpha-melanocyte-stimulating hormone (alpha-MSH)

All these studies have demonstrated that MSCs can enhance peripheral nerve regeneration via two mechanisms: 1) MSCs differentiate into functional schwann cells, therefore providing myelination and axon sprouting support