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The animals were placed in the stereotaxic device (David Kopf Instruments, Tujunga, CA, USA) and holes were drilled in the frontal, parietal, and occipital regions of the skull at the following locations: 2 mm from Bregma, 2 mm lateral to the midline (ipsilateral), and 2 mm posterior to Lambda (reference electrode)

Mechanisms of poor oral bioavailability of flavonoid morin in rats: from physicochemical to biopharmaceutical evaluations

Then 100 L of supernatant was collected in a fresh plate

In addition, elevating miR-722 diminishes ROS generation, as evidenced by decreased production of hydrogen peroxide in neutrophils (39)

Miller JW