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For transfection of Lenti-X 293T, cells were seeded in the 6-well plate on the first day and transfected on the second day when the cells reached approximately 70%90% confluent, as described previously ( Confocal fluorescence microscopy analysis Lenti-X 293T cells were seeded on cell slides in 24 well-plate on the first day and transfected on the next day when cells reached 30%50% confluence

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Beads were transferred to a gravity flow column, washed with 40 column volumes of lysis buffer and eluted with 5 column volumes of lysis buffer containing 500 mM imidazole

AAPS J 15(1):195218 Hashem S, Nisar S, Sageena G, Macha MA, Yadav SK, Krishnankutty R, Uddin S, Haris M, Bhat AA (2021) Therapeutic effects of curcumol in several diseases