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The nuclei were stained at RT for 10 min without light by 4, 6-diamidino-2-phenylindole (DAPI)

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This regulatory effect depends on a critical signaling axis involving the inhibition of ERK1/2 phosphorylation and subsequent downregulation of hypoxia-inducible factor-1 expression, which constitutes the central molecular mechanism through which inosine modulates B cell function [60]