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Application of this assay to analyze plasma from patients with known oxidative stress (sickle cell disease and sepsis) and from a patient with sickle cell disease treated with the antioxidant N-acetylcysteine suggests that cysteine disulfides, in particular protein-bound cysteine, serve as sensitive plasma biomarkers for the extent of oxidative stress and effectiveness of antioxidant treatment

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In ALS populations, change in HHD scores was correlated with ALSFRS-R and vital capacity (VC), with coefficient of variation performing better than VC, but not as well as ALSFRS-R [47]