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J.MekleR.et al

SAHH ( S -adenosylhomocysteine hydrolase)-coupled assay showed that MS2734 inhibited NNMT at a median inhibitory concentration (IC50) of 14 1.5 m (102)

Denatured proteins are precipitated by cooling and removed via centrifugation, keeping of NAD + , NADH, NADP + , NADPH, GSSG and GSH stable in the supernatant