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Cell 85 , 465467 (1996)

Dysregulation of redox homeostasis leads to ROS accumulation, mitochondrial dysfunction, disrupted iron metabolism, and DNA damage, ultimately contributing to oxidative stress [168170]

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Many of the published papers report the use of NycoPrep 1.15, an isoosmotic solution of 27.6% (w/v) Nycodenz in 3 mM KCl, 0.3 mM CaNa2-EDTA 5 mM Tris-HCl, pH 7.5