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Basal cells were defined as ITGA6-positive cells and suprabasal cells as ITGA6-negative cells

The protocol for immobilization of GST-SPINK3 to glutathioneagarose beads was modified from previously reported protocols by using an alternative bifunctional cross-linker (dithiobis(succinimidyl propionate)) in a very simple procedure and by using simple buffers under physiological conditions