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Amersham, UK) (20), using mini trans-blot apparatus (Bio-Rad, Hercules, CA) following the manufacturers instructions

For instance, affinity chromatography is reputed for its high affinity, but tightly bound proteins typically require relatively extreme eluting conditions to disassociate from affinity columns

Arch Biochem Biophys 126:7582 Malfatto G, Facchini M, Branzi G, Riva B, Sala L, Perego GB (2003) Long-term treatment with the beta-blocker carvedilol restores autonomic tone and responsiveness in patients with moderate heart failure

A similar reduction occurred in the presence of anti-CD44 KM114 monoclonal antibody, which masks the hyaluronic acid-binding site, whereas an irrelevant human TRAIL (hTRAIL)Fc fusion had no effect (Fig

[DOI] [PMC free article] [PubMed] [Google Scholar] 388.Akhtar M.S., Panwar J., Yun Y.S