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For RNA extraction, cells were washed twice with cold PBS (without calcium and magnesium) and harvested, and upon RNA analysis at the 12-well format, cells were exposed to QIAzol reagent (see below) and further processed

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While such a limited dataset did not allow confirmation of the accuracy of the PK/PD relationship, clear dose/exposure-dependent target engagement, as shown by the ratio between Met-EF1 level at C2D1 compared to the level at screening, was observed [26]

NNMT interacts with nicotinamide metabolism, leading researchers to investigate potential downstream effects on NAD availability