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Caffeinated coffee has long been thought to be good for the liver and linked to slowing down liver scarring in various liver diseases

- Nutritional support to further reduce cravings

Procedure for sulfhydryl modification required reaction of rat anti-mouse CD5 or isotype IgG antibodies (Biolegend, San Diego, CA, USA) with 20-fold excessive SATA (N-succinimidyl S-acetylthioacetate) (Millipore Sigma) at room temperature for 30 min

After filtration with 0.45 m filters to remove cell debris, the medium was concentrated at 4,000 g for 1 hour by using 3 KD Ultra Centrifugal Filters (Millipore) to a desired volume

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