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Isolation of cell membrane from macrophage RAW 264.7 cells were obtained from the American Type Culture Collection (ATCC) and maintained in Dulbeccos modified Eagle medium (DMEM, Corning) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillinstreptomycin (Beyotime)

[DOI] [PubMed] [Google Scholar] 33.Briones A.M., Touyz R.M

Freshly dissected rat corpus cavernosum tissues were rinsed and vigorously agitated in ice-cold PBS to remove residual blood cells, followed by mincing and enzymatic digestion with 2.5 mg/ml collagenase type I, 4 mg/ml collagenase type IV, 0.1 mg/ml neutral protease, and 2 mg/ml DNase I at 37C for 40 min[31]

Consistent with Glut1 expression patterns, Th1, Th2, and Th17 cells exhibit increased rates of glycolysis, while Treg cells exhibit increased rates of fatty acid oxidation