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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

Other Ways To Lower The Risk Of ISS There are other ways you can work with your veterinarian to help lower the risk of injection-site sarcoma in your cat

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This equalizes pressure inside the vial and makes drawing the liquid easier

Nigro DA, Wein AJ, Foy M et al: Associations among cystoscopic and urodynamic findings for women enrolled in the interstitial cystitis data base (icdb) study