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discussion S64-73

eLife 6:e24845

Multiple molecular mechanisms have been implicated in PD pathogenesis, including mitochondrial dysfunction, oxidative stress, impaired protein degradation pathways (ubiquitin-proteasome and autophagy-lysosomal systems), excitotoxicity, calcium dysregulation, neuroinflammation, and abnormal alpha-synuclein aggregation

coli GI826 competent cells, which were then cultured in 50 ml of IMC medium (1 M9 salts, 0.2% casamino acid, 0.5% glucose, 1 mM MgCl 2 ) containing 100 g/ml ampicillin with shaking (225250 rpm) to saturation (OD 600 = 3) for 15 hours at 25C

These findings were replicated when SLC7A11 expression was directly manipulated (Supplementary Fig