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Mahmoud NN, Hamad K, Al Shibitini A, Juma S, Sharifi S, Gould L, et al

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To visualize H 2 O 2 -driven PDI oxidation by GPx7 and the luminal domain of GPx8, reduced PDI (10 M) was reacted with GPx7 or GPx8 (1 M each) in the presence of 10, 50 or 200 M of H 2 O 2 , and the redox-state change of PDI was monitored by mal-PEG 2K modification of free cysteines followed by non-reducing SDS-PAGE (Fig

Mutations in these genes amongst the most common genetic causes of familial PD disrupt this surveillance system, permitting damaged mitochondria to accumulate and generate neurotoxic levels of ROS [13]