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10.1126/science.aan4183 Science 5 ChanW.AlmasiehM.CatrinescuM.-M.LevinL

Cell culture and measurement of cellular NAD + CHO cells stably transfected with a mouse CD38 plasmid (CHO-mCD38) were cultured in DMEM medium supplemented with 10% FBS (Thermo Fisher Scientific), 2 mM L-glutamine, 120 U ml 1 penicillin and streptomycin (Lonza, Alpharetta, GA), and 400 nM nicotinamide mononucleotide (NMN))

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The hCG diet combines daily doses of human chorionic gonadotropin (hCG)a hormone produced during pregnancywith an extremely restrictive 500-calorie daily intake