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Sephacryl S-300 size exclusion chromatography Fractioning the GST containing concentrate on a Sephacryl S-300 column (142 cm 1.75 cm) pre-equilibrated and run with 0.02 M Na-phosphate buffer pH 7.0 at 30 ml/h flow rate with collection of 2 ml fractions

A lack of conjugation between the A and B rings usually results in decreased antioxidant activity, and the absorption spectra are reflected by an intense band II and only a weak shoulder for band I (Jomova et al

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E.McSorleyD.et al (1999)