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Crosstalk between butyrate oxidation in colonocyte and butyrate-producing bacteria

doi.org/10.1007/978-3-030-48798-0_8

Identification of molecular species of glycerophospholipids and sphingomyelin using electrospray mass spectrometry

The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway 1

Briefly, the coding sequence (CDS) of HrGSTm1 was cloned into the pET-32a(+) vector (TaKaRa, Shiga, Japan) with Bam HI and Hin dIII restriction sites, then transformed into competent Escherichia coli (DE3) cells (AxyGen, Shanghai, China) and cultured at 37 C for 24 h