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After centrifugation, add 2.5 L 2 kinase/metal ion solution 384 assay plate, and incubate at 25 C for 10 min

Aging 44, 225245

After washing, the membranes were incubated with corresponding HRP-conjugated secondary antibodies (1:5,000) at 25C for 1.5 h

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Cardiovascular diseases in which L-Arg supplementation therapy has been tested are associated with oxidative stress and oxidative depletion of the essential eNOS cofactor BH 4 , causing eNOS uncoupling