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5 Methods 5.1 Cell culture The THP-1 human leukemic monocytic cell line, obtained from the American Type Culture Collection, was cultured in RPMI-1640 medium (R0883-500ML, Sigma-Aldrich) supplemented with 10% heat-inactivated FCS, 2 mM L-glutamine, and 100 U/ml penicillin-streptomycin, and incubated at 37C in a 5% CO 2 atmosphere

Long-term combined-stack outcomes beyond the trial windows are unstudied

Everyday Examples: Cold Weather Survival Time Applying the previous equation to the human body we can estimate how long it would take body temperature to drop from a normal 37 C down to the edge of moderate hypothermia at 33 C in the example survival situations discuss so far in this unit

The peptide also stimulates collagen and elastin production, enhances fibroblast activity, and promotes organized tissue remodeling

After adjustment in Model I, the coefficient was 0.006 (95% CI -0.027 to 0.016, p = 0.607)