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Description

The inherent pathological changes of stroke have been shown to be closely related to the characteristics of ferroptosis, including iron metabolism disorders, lipid peroxidation, and elevated ROS levels

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Glutathione depletion up-regulates Bcl-2 in BSO-resistant cells

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For experiments requiring manipulation of cystine availability, cells were cultured in RPMI 1640 medium without L-glutamine, L-cysteine, L-cystine, and L-methionine (Fisher Scientific, ICN1646454) supplemented with 5% dialyzed FBS (Sigma-Aldrich, F0392), L-glutamine (VWR, VWRL0131-0100), and L-methionine (Sigma-Aldrich, M5308)