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While these wash steps are pretty easy, one important point to keep in mind is that you will want to use a specific amount of contact time

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These studies implicate mitochondrial metabolic programming in microglial activation and suggest that perturbations in mitochondrial energy homeostasis may enhance pro-inflammatory microglial phenotypes associated with AUD and AD (Rose et al., Mitochondria also generate ROS primarily through oxidative phosphorylation (OXPHOS) where the majority of ATP is produced (Figure 1)