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The genotype of the mice was confirmed by sequencing the PCR fragments, with the following PCR primers: TCGAAGGTTTCCCAGTGAGC for Flox-F1 (P4hb-Flox-F1), and AGATCCACCAGCCTCTACCC for Flox-R1 (P4hb-Flox-R1)

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10.1513/AnnalsATS.201503-133AW Ann Am Thorac Soc

This patient's normal MEPP and normal response to applied acetylcholine confirm intact postsynaptic receptor function, making a prolonged pancuronium effect unlikely